Invitrogen™ SNARF™-5F 5-(and-6)-Carboxylic Acid
On demand
Order number :
S23922
Invitrogen™ SNARF™-5F 5-(and-6)-Carboxylic Acid
The cell-impermeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid (pKa of ∼7.2) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
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