Invitrogen™ Streptavidin, R-Phycoerythrin Conjugate (SAPE) - premium grade - 1 mg/mL

Invitrogen™ Streptavidin, R-Phycoerythrin Conjugate (SAPE) - premium grade - 1 mg/mL

The R-phycoerythrin (R-PE) conjugate of streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (R-phycoerythrin). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Streptavidin R-PE is very valuable in biotin-streptavidin detection protocols developed for flow cytometry, microplate assays, and microarrays. This R-PE streptavidin conjugate is a high-purity product (purity confirmed by HPLC) and is supplied as 1 mL of a 1 mg/mL solution.

Features

• Ex/Em maxima of ∼ (496, 546, 565/578) nm
• 5 to 10 times brighter than organic dyes
• Very high solubility in aqueous solutions
• Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE
• Ideal for flow cytometry, microplate assays, and microarrays