Thermo Scientific™ Pierce™ Recombinant Protein A/G, 5 mg
On demandThermo Scientific™ Pierce™ Recombinant Protein A/G, 5 mg
Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G.
Features
- Contains four Fc-binding domains from Protein A and two from Protein G
- Provides binding for all antibody species and subclasses recognized by either Protein A or Protein G
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