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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit, 500 Reactions

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 500 Reactions
Order number : 11750350
(Ex-work price)
$ On demand

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit, 500 Reactions

 

The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

 

Features

  • Compatible with a wide range of mammalian cell types grown under different treatment conditions
  • Single-tube format minimizes reagent loss, sample loss, and handling time
  • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
  • High-quality cDNA generated for use in a variety of applications, including PCR and qPCR
  • Simple protocol takes around 37 minutes
  • Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers
  • Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA

 

Contents

  • SuperScript IV CellsDirect Lysis Solution, 9 mL
  • SuperScript IV CellsDirect Stop Solution, 1.25 mL
  • Lysis Enhancer, 250 µL
  • Dnase I, 500 µL
  • SuperScript IV RT Master Mix, 1 mL
  • SuperScript IV No RT Control, 1mL


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