Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit, 400 Reactions

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit, 400 Reactions

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. SYBR Green Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Cells-to-Ct Fast Advanced reverse transcription enzyme mix, 2X RT buffer, and best-in-class PowerUp SYBR Green Master mix. The SYBR Green Fast Advanced Cells-to-Ct Kit gives you the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 1.5 hours.

Features

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new PowerUp SYBR Green Fast Advanced Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 1000 real-time PCR results from 100 starting samples

Contents

• 20X Fast Advanced RT Enzyme Mix: 1 mL
• 2X Fast Advanced RT Buffer: 20 mL
• Lysis Solution: 20 mL
• Stop Solution: 1 mL
• DNase I:  220 µL
• PowerUp SYBR Green Master Mix: 20 mL