Invitrogen™ LIVE/DEAD™ Sperm Viability Kit

Invitrogen™ LIVE/DEAD™ Sperm Viability Kit

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR™ 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant propidium iodide labels the nucleic acids of membrane-compromised sperm with red fluorescence.

Contents

• SYBR 14 dye (Component A), 100 μL of a 1 mM solution in DMSO.
• Propidium iodide (Component B), 5 mL of a 2.4 mM solution in wate.