Invitrogen™ T4 DNA Polymerase, 250 Unites

Invitrogen™ T4 DNA Polymerase, 250 Unites

T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available.

Applications: Labeling double-stranded linear DNA by replacement synthesis. Oligonucleotide-directed, site-specific mutagenesis. 3´ end-labeling of double-stranded DNA. Polishing both 5´ or 3 overhangs to make blunt ends.

Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid.

Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested.

Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template•primer.

Contents

• T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.