Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells, 20 Reactions

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells, 20 Reactions

The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. The TA Cloning® Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

Features

• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening and >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites

Contents

• Linearized pCR™2.1 vector
• ExpressLink™ T4 DNA ligase
• 5X ExpressLink™ T4 DNA ligation buffer
• dNTPs, 10X PCR buffer
• Sterile water
• Controls