Product Image Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

The pENTR™ 5’-TOPO® TA Cloning® Kit combines Invitrogen’s TOPO® Cloning and MultiSite Gateway® Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway® entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO® vector to a suitable MultiSite Gateway® destination vector in a MultiSite Gateway® LR recombination reaction with other suitable entry clone(s) to generate an expression construct.

Features

• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells
• Rapid PCR cloning with TOPO® cloning technology for cloning the 5’ element
• attL4 & attR1 sites for compatibility with MultiSite Gateway® LR reaction for increased flexibility

Contents

• The TOPO™ Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO™ vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. 
• The One Shot™ TOP10 box contains transformation reagents including 20 single-use 50 µl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control.