Invitrogen Tau (Phospho) [pS199] Mouse ELISA Kit, 96 Tests

Invitrogen Tau (Phospho) [pS199] Mouse ELISA Kit, 96 Tests

The Mouse Tau (Phospho) [pS199] ELISA quantitates Ms Tau (Phospho) [pS199] in mouse cell lysates, cell culture medium and brand homogenates. The assay will exclusively recognize both natural and recombinant Ms Tau (Phospho) [pS199].
Principle of the method
The Ms Tau (Phospho) [pS199] solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 3.8%
• Intraassay CV: 5.6%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Ms Tau [pS199] Standard, lyophilized. Refer to vial label for reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 27 mL
• Antibody Coated Plate, 96-well plate, 1 plate
• Ms Tau [pS199] Biotin Conjugate (100X), 0.120 mL
• Streptavidin-HRP (100X), 0.150 mL
• Assay Buffer (20X), 5 mL
• Wash Buffer Concentrate (20X), 50 mL
• Substrate Solution, Tetramethylbenzidine (TMB), 15 mL
• Stop Solution, 15 mL
• Plate Covers, adhesive strips, 4