Invitrogen Tau (Total) Human ELISA Kit, 96 Tests

Invitrogen Tau (Total) Human ELISA Kit, 96 Tests

The Human Tau (Total) ELISA quantitates Hu Tau in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau, independent of phosphorylation state.

Principle of the method
The Human Tau solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 6.5%
• Intraassay CV: 4.6%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Human Tau (Total) Standard, recombinant Human Tau-352 expressed in E. coli; contains 0.1% sodium azide. Refer to vial
• label for reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Human Tau Antibody Coated Plate, 96-well plate, 1 plate
• Human Tau Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin-HRP (100X), 0.15 mL
• Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 3