Invitrogen TIE2 Human ELISA Kit, 96 Tests
On demandInvitrogen TIE2 Human ELISA Kit, 96 Tests
The Human Tyrosine Kinase Receptor (Hu TIE-2) ELISA quantitates Hu TIE-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIE-2.
Principle of the method
The
Human TIE-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 6.3%
- Intraassay CV: 7.1%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Controls
- Adhesive Plate Covers
There are no specifications
There are no report
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