Invitrogen AKT/ERK/p70 S6K Activation Multispecies InstantOne™ ELISA Kit, 96 Tests

Invitrogen AKT/ERK/p70 S6K Activation Multispecies InstantOne™ ELISA Kit, 96 Tests

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), AKT 1/2/3 (human, mouse, hamster), and p70 S6K (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Features

• Homogenous (no wash): No
• Interassay CV: 5.2%
• Intraassay CV: 4.7%
• Storage: 2-8°C

Contents

• User Manual
• phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Capture Antibody Reagent (Part No. IOPERKCAR1T) - 1 mL
• phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Detection Antibody Reagent (Part No. IOPERKDAR1T) - 1 mL
• phospho-AKT 1/2/3 (Ser437) Capture Antibody Reagent (Part No. IOPAKTACAR1T) - 1 mL
• phospho-AKT 1/2/3 (Ser437) Detection Antibody Reagent (Part No. IOPAKTADAR1T) - 1 mL
• phospho-p70 S6K (Thr389) Capture Antibody Reagent (Part No. IOPS6KCAR1T) - 1 mL
• phospho-p70 S6K (Thr389) Detection Antibody Reagent (Part No. IOPS6KDAR1T) - 1 mL
• InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed)
• Cell Lysis Buffer (5X) (Part No. IOCLB1) - 12 mL
• Enhancer Solution (Part No. IOES1) - 1 mL
• Wash Buffer (10X) (Part No. IOWB1) - 15 mL
• Detection Reagent (TMB Substrate) (Part No. IODR1) - 12 mL
• Stop Solution (Part No. IOSS1) - 12 mL
• Positive Control Cell Lysate (lyophilized) (Part No. IOMEAPPC1) – 0.25 mL
• Plate seal - 1 ea