Invitrogen TNFRSF9 Human ELISA Kit, 96 Tests

Invitrogen TNFRSF9 Human ELISA Kit, 96 Tests

The Human TNFRSF9 ELISA quantitates Hu TNFRSF9 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFRSF9.

Principle of the method

The Human TNFRSF9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: <12%
• Intraassay CV: <10%
• Homogenous (no wash): No
• Storage: 2-8°C

Contents

• Human TNFRSF9 Antibody Coated wells, 96-well plate, 1 plate
• Human TNFRSF9 Biotin Conjugate, 2 vials
• Human TNFRSF9 Standard, recombinant human TNFRSF9, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (600X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2