Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit

Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit

The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

Features

Benefits of the TrueTag Knockout Enrichment Donor DNA Kit include:

• Easily design and order all necessary components to generate a knockout cell line
• Save up to 3 months with enrichment compared to single cell isolation and characterization
• Leverage a selection marker to obtain up to 100% edited cells

The TrueTag Knockout Enrichment Donor DNA Kit provides everything you need to achieve functional gene knockout and enrich the edited cells. The donor produced will insert 3X STOP codons, a selection marker, and a fluorescent tag—simply provide two locus-specific primers to add homology arms and PCR-amplify the donor. Each kit is designed to enable you to:

• Insert 3X STOP codons to knock out gene function
• Tag each allele with a different fluorophore (GFP or RFP) to verify bi-allelic knockout
• Utilize puromycin and blasticidin selection markers for bi-allelic knockout confirmation
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit

• Four linear donor templates: GFP/puromycin resistance, GFP/blasticidin resistance, RFP/puromycin resistance, RFP/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers for knockout of human HPRT1