Invitrogen VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit, 96 Tests
On demandInvitrogen VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit, 96 Tests
The Mouse Vascular Endothelial Cell Growth Factor Receptor 2 (VEGF R2/VEGFA) ELISA quantitates Ms VEGF R2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VEGF R2.
Principle of the method
The
Mouse VEGF R2 solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Storage: 2-8°C
- Shipping condition: Wet or Dry Ice
Contents
- Mouse VEGF R2 Antibody Coated wells, 96-well plate, 1 plate
- Mouse VEGF R2 Biotin Conjugate 2 vials
- Mouse VEGF R2 Standard, recombinant mouse VEGF R2, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent C, 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (300X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2