Invitrogen™ Vybrant™ DyeCycle™ Green Stain

Invitrogen™ Vybrant™ DyeCycle™ Green Stain

Vybrant™ DyeCycle™ Green Stain is a cell-permeable DNA dye that can be used for cell cycle analysis on a 488 nm spectral line of the argon-ion laser, which is commonly used on all flow cytometers.

Features

• Precise, Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass.
• Low Cytotoxicity, Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
• Simple, Robust Staining Protocol, For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 10⁶ cells/mL. To each tube add 2 µL of Vybrant™ DyeCycle™ Green Stain. Final stain concentration is 10 µM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.

Contents

• 1 vial of Vybrant™ DyeCycle™ green stain (5 mM in DMSO).