Invitrogen™ Vybrant™ DyeCycle™ Ruby Stain, 400 reactions
On demand
Order number :
V10273
Invitrogen™ Vybrant™ DyeCycle™ Ruby Stain, 400 reactions
Vybrant® DyeCycle™ Ruby Stain is a cell permeable DNA dye that can be used for cell cycle analysis on a 488 nm or 635 nm red diode laser.
Features
- Precise, Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Ruby Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass.
- Low Cytotoxicity, Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant® DyeCycle™ Ruby Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
- Minimal Compensation, Well-suited
for the popular red 633 nm laser line, the Vybrant® DyeCycle™ Ruby
Stain/DNA complex has fluorescence excitation and emission maxima of
638/686 nm, respectively. The spectral characteristics
allow use of the red laser for cell cycle analysis and frees up other
channels for additional parameters.
The red excitation and narrow emission of Vybrant® DyeCycle™ Ruby Stain make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor® 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP)). - Simple, Robust Staining Protocol, For cell analysis, simply prepare flow cytometry tubes containing 0.5 mL of cells suspended in complete media at a concentration of 5 × 105 cells/mL. To each tube, add 1 µL of Vybrant® DyeCycle™ Ruby Stain and mix well. Final stain concentration is 5 µM. Incubate at 37°C for 15–30 minutes, protected from light. Analyze without washing cells on a flow cytometer using 488 nm or 633/5 nm excitation and >670 nm emission.
Contents
- 1 vial of Vybrant™ DyeCycle™ ruby stain (2.5 mM in DMSO).
There are no specifications
There are no report
You May Also Like
Invitrogen™ Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for Flow Cytometry
(Ex-work price)
$ On demand
$ On demand
On demand
Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, SYTOX Green, R-PE, 50 Assays
(Ex-work price)
$ 1,014
$ 1,014
On demand
Invitrogen™ LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation, 400 Assays
(Ex-work price)
$ On demand
$ On demand
On demand
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506, 500 Tests
(Ex-work price)
$ On demand
$ On demand
On demand
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack
(Ex-work price)
$ On demand
$ On demand