Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit, FITC, Propidium Iodide, 100 Tests
On demandInvitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit, FITC, Propidium Iodide, 100 Tests
Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).
Contents
- Binding Buffer (4X), 1 bottle (50 mL).
- rh Annexin V-FITC, 1 vial (0.5 mL).
- Propidium Iodide (20 µg/mL), 1 vial (1.8 mL).
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/68cdbc0c-2f1c-45eb-9dc4-2440ce56f2a9.webp)
Invitrogen™ CellMask™ Deep Red Actin Tracking Stain, 1 x 60 assays
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/f382f84e-bbce-458c-9d0c-dc6cd31a2a42.png)
Invitrogen™ LIVE/DEAD™ Fixable Olive (557) Viability Kit, for 488 nm Excitation, 400 Assays
$ 649
On demand
![](https://laboshop.ae/public/storage/files/0949ca87-1495-4774-b331-a2b67f28b43e.jpg)
Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/0bd80c13-b4c5-431d-9bef-20faddfe82c9.webp)
Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/e4zMvwv6YpdI5WPDasCVpeqxbxScqSrKgNEvm7DD.png)
Invitrogen™ Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for Flow Cytometry
$ On demand