Invitrogen™ Zenon™ Mouse IgG2b Labeling Kits, Alexa Fluor™ 488
On demandInvitrogen™ Zenon™ Mouse IgG2b Labeling Kits, Alexa Fluor™ 488
Quickly generate antibody isotype conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Mouse IgG2b Labeling Kits. Simplify your laboratory applications and reduce antibody cross-reactivity while also achieving efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.
Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled IgG primary antibodies with the Zenon™ Mouse IgG2b labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.
Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.
Features
- Labeled antibodies are typically ready to use in 10 minutes
- Requires only 1–20 µg primary antibody
- Simple—no purification required
- Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase
- Multiplex with other mouse monoclonal antibodies simultaneously
- Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.
Contents
- Contains 1 vial of Zenon™ Alexa Fluor™ 488 mouse IgG2b labeling reagent (250 µL), and 1 vial of Zenon™ blocking reagent (mouse IgG, 250 µL)
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