Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit, 1000 Reactions

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit, 1000 Reactions

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

For use with: Luminometer (Microplate)

Features

• Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
• Cost effective—highest sensitivity assays result in decreased reagent consumption
• Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
• Time-saving—assays using secreted luciferases require minimal sample handling
• Automation-friendly—amenable to high throughput screens
• Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
• Safe—allows one to perform non-radioactive assays

Contents

• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)
• 100X Coelenterazine, 0.5 mL (store at -80°C)
• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)