Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents, pHrodo™ iFL Green, Mouse

Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents, pHrodo™ iFL Green, Mouse

Achieve rapid, scalable, and efficient labeling of internalized antibody with the Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents. These pH-sensitive dye conjugates are non-fluorescent outside the cell but fluoresce brightly red or green when inside acidic environments, including those of phagosomes. As a result, pHrodo iFL IgG Labeling Reagents can offer faster and more accurate results than any other phagocytosis assay.

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Features

• Simplicity — No reagent purification is required
• Accuracy — No wash steps or quenchers are needed, minimizing cross-reactivity or background
• Speed — Labeled antibodies are typically ready to use in 10 minutes
• Specificity — Dyes are designed for the specific detection of phagocytosis and endocytosis

Contents

• Contains 1 vial of Zenon™ pHrodo™ iFL Red mouse labeling reagent (250 µL).