Thermo Scientific™ DTBP (Wang and Richard's Reagent)
On demandThermo Scientific™ DTBP (Wang and Richard's Reagent)
Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
Dimethyl dithiobispropionimidate (DTBP) is a homobifunctional, cleavable and membrane permeable crosslinker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.
Features
- Reactive groups: imidoester (both ends)
- Reactive towards: amino groups (primary amines)
- Imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
- Cleavable by reduction of spacer arm disulfide bond with DTT, TCEP or other reducing agents
- Compare to DMS (non-cleavable)
- Water-soluble
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