Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli, 25 Reactions
On demand
Order number :
K288020
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli, 25 Reactions
The Zero Blunt® TOPO® PCR Cloning Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ('TOPO® Cloning') for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4Blunt-TOPO® vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice.
Features
- Get More Sequence—Allows for more insert sequence and less vector sequence when using standard sequencing primers
- Fast and Easy—Go from PCR-to-clones in just 3 steps and in as little as 5 minutes hands-on time
- Efficient—Achieve up to 95% clones with correct insert
- Proven—Reliable performance for over a decade with over 4000 citations
Contents
Box 1:
- Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
- Salt solution
- dNTPs
- Control template
- T3, T7, M13 forward, and M13 reverse primers
- Control PCR primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid
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