Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit
On demand
Order number :
K89020
Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit
The pIB/V5-His TOPO® TA Expression Kit offers five-minute cloning of Taq-amplified PCR products directly into the pIB/V5-His-TOPO® expression vector. In addition to being TOPO® Cloning ready,
pIB/V5-His-TOPO® includes:
- The OpIE2 promoter for constitutive expression
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
Contents
- The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector
- dNTPs
- 10X PCR buffer
- control PCR template and primers
- forward and reverse primers for sequencing and PCR screening
- salt solution
- pIB/V5-His/CAT.
- The One Shot™ box contains high-efficiency single-use
- 50 µL aliquots of chemically competent TOP10 E. coli cells
- S.O.C. medium
- pUC19 supercoiled plasmid control.
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