Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
On demand
Brand origin :
China
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
500
Order number :
K283020
Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector to allow easy, high-efficiency cloning of blunt-end PCR products.
Features
- Unique technology to minimize background
- Get your clone—95% efficiency
- Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
- Kanamycin and Zeocin resistance genes for your choice of selection
- Convenient, validated restriction sites in MCS for further subcloning
- Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
Contents
Box 1:
- Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid
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