Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

On demand
Brand origin : China
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 500
Order number : K283020
(Ex-work price)
$ On demand

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector to allow easy, high-efficiency cloning of blunt-end PCR products.


  • Unique technology to minimize background
  • Get your clone—95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
  • Kanamycin and Zeocin resistance genes for your choice of selection
  • Convenient, validated restriction sites in MCS for further subcloning
  • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time


Box 1:

  • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Sterile water

Box 2:

  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid


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