Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
On demand
Order number :
K240020
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
The pENTR™/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or restriction enzymes required.
Features
- Gateway® System Ready—Rapidly shuttle cloned genes between multiple vector systems
- Fast and Easy—Go from PCR to Gateway® Entry clone in just 3 steps and as little as ∼5 minutes hands on time
- Efficient—Achieve over 90% clones with correct insert in the right direction
- Proven—Reliable performance for over a decade
Contents
- pENTR⁄D-TOPO™ vector
- dNTPs
- Salt solution
- Sterile water
- Universal M13 sequencing primers
- OneShot™ TOP10 Chemically E. coli
- S.O.C. Medium
- pUC19 control plasmid.
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