Invitrogen™Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate

 Invitrogen™Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate

This is the Galacton-Star® chemiluminescent substrate solution and substrate dilution buffer for the detection of E. coli β-galactosidase for use with the Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System.

Features

• Compatible Cells: Mammalian Cells
• High-throughput Compatibility: High-throughput Compatible
• Readout: End Point
• Target Enzyme: Beta-Galactosidase
• Technique: Enhanced Chemiluminescence
• Form: Liquid
• Product Line: Galacto-Star™, Galacton-Star™
• Validated Application:Enzyme Assay
• Product Type: Reaction Buffer Diluent with Substrate
• This product contains the substrate and buffer diluent. Store the product at 4°C.

Contents

• Microplate assays per kit 
• Lysis Solution : 00 mM potassium phosphate (pH 7.8), 0.2% Triton X-100 (for mammalian cells, in T1012, T1014, T1013)
• OR 5X Z Buffer: 0.5 M sodium phosphate (pH 7.1), 50 mM KCl, 5 mM MgSO4 (for yeast cells, in T1019, T1020). 
• Galacton-Star substrate: 50X concentrate 
• Reaction Buffer Diluent: 100 mM sodium phosphate (pH 7.5), 1 mM magnesium chloride, 5% Sapphire-II enhancer.