Invitrogen™ MitoProbe™ JC-1 Assay Kit

Invitrogen™ MitoProbe™ JC-1 Assay Kit

The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry.
The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (∼529 nm) to red (∼590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates.
Unlike other mitochondrial stains that are affected by plasma membrane potential, the ratio of green to red fluorescence of JC-1 depends only on the membrane potential and not other factors such as mitochondrial size, shape, and density, which may influence single-component fluorescence signals. Use of fluorescence ratio detection allows researchers to make comparative measurements of membrane potential and determine the percentage of mitochondria within a population that respond to an applied stimulus.

Features

• Excitation/Emission: 514⁄529, 590
• Flow Cytometer Laser Lines: 488
• Label Type: Other Label(s) or Dye(s)
• Label or Dye: JC-1
• Detection Method: Fluorescent
• Sub Cellular Localization: Mitochondria
• For Use With (Equipment): Flow Cytometer
• No. of Reactions: 100

Contents

• JC-1 (5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcar-bocyanine iodide): 5 vials each containing 30 μg powdered dye
• DMSO: 1.5 mL dimethlysufoxide
• CCCP: 125 μL of 50 mM CCCP in DMSO
• 10X phosphate-buffered saline: 25 mL