Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit, 500 Reactions

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit, 500 Reactions

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features

• Efficiency—offers significant time and cost savings for increased throughput
• Convenience—enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
• Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
• For Use With (Equipment): 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ

Contents

• Resuspension Buffer:  10 mL
• Lysis Enhancer: 1 mL
• DNase I, Amplification Grade (1 U/µl):  2 × 1.25 mL
• 10X DNase I Buffer:  800 µL
• 25 mM EDTA:  2 × 1 mL
• SuperScript™ III RT/Platinum™ Taq Mix:  500 µL
• 2X Reaction Mix:12.5 mL
• 50 mM MgSO4:1 mL  
• DEPC-treated water:  12.5 mL
• HeLa Total RNA (10 ng/µl):  10 µL
• ROX™ Reference Dye:  500 µL