Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit

Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit

The ChargeSwitch® Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 ml of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 µg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.
The ChargeSwitch® Plasmid ER (Endotoxin Reduced) Mini Kit allows rapid and efficient purification of highly pure plasmid DNA from E. coli cells. After preparing cell lysates, the plasmid DNA can be purified from lysates in less than 10 minutes using a novel magnetic bead-based technology that uses magnetic beads conjugated to a unique ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Yields of up to 20 µg plasmid DNA are typically obtained from 1-5 ml of fresh, overnight bacterial cultures without the use of chaotropic salts, ethanol, organics or isopropanol precipitation, making it ideal for mammalian transfection. ChargeSwitch® Plasmid ER Mini Kit includes Endotoxin Reduction Reagent (ETRR) that substantially lowers the endotoxin levels (< 50 EU/ µg DNA) in the purified plasmid DNA.

Features

• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
• Final Product Type: Plasmid DNA
• Purification Target: Plasmid DNA
• Sample Type: Bacterial Culture
• Prep Scale: < 100 µg (Small-Scale) Plasmid DNA
  
  

Contents

• ChargeSwitch® Magnetic Beads (25 mg/ml): 2 x 1 mL
• RNase A: 0.4 mL
• Resuspension Buffer: 15 mL
• ChargeSwitch® Lysis Buffer (L9): 15 mL
• ChargeSwitch® Precipitation Buffer (N5): 15 mL
• ChargeSwitch® Wash Buffer (W11): 50 mL
• ChargeSwitch® Wash Buffer (W12): 50 mL
• ChargeSwitch® Elution Buffer: 10 mL
• Endotoxin Reduction Reagent (D1; ETRR): 4.5 mL