Invitrogen™ Chargeswitch™ Pro Filter Plasmid Maxi Kit, 25 Preps

Invitrogen™ Chargeswitch™ Pro Filter Plasmid Maxi Kit, 25 Preps

The Chargeswitch® -Pro Filter Plasmid Maxi Kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch®-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification" column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding" column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.


Features

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (K211101)
• Starting Material Volume: 100 mL
• Elution Volume Max: 2 mL
• Elution Volume Min: 1 mL
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: > 200 µg (Large-Scale) Plasmid DNA
• Purity or Quality Grade: Transfection Grade
• Final Product Type: Plasmid DNA
• Purification Target: Plasmid DNA
• Sample Type: Bacterial Culture

Contents

• ChargeSwitch®-Pro Resuspension Buffer: 185 mL
• ChargeSwitch®-Pro Lysis Buffer: 185 mL
• ChargeSwitch®-Pro Precipitation Buffer: 185 mL
• ChargeSwitch®-Pro RNase A: 0.95 mL
• ChargeSwitch®-Pro Wash Buffer 1: 190 × 2 mL
• ChargeSwitch®-Pro Wash Buffer 2: 190 × 2 mL
• ChargeSwitch®-Pro Elution Buffer: 50 mL
• ChargeSwitch®-Pro Filter Plasmid Maxi Columns: 25 (CS32000)