Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit, 10 Preps

Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit, 10 Preps

The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients.
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.


Features

• Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product—pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
• Final Product Type: BAC DNA, Plasmid DNA
• Purification Target: BAC DNA, Plasmid DNA
• Sample Type: Bacterial Culture
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: > 200 µg (Large-Scale) Plasmid DNA

Contents

• Resuspension Buffer (R3): 100 mL
• RNase A: 550 μL
• Lysis Buffer (L7): 100 mL
• Precipitation Buffer (N3): 100 mL
•  Equilibration Buffer (EQ1): 300 mL
• Wash Buffer (W8): 2 × 300 mL
• Elution Buffer (E4): 30 mL
• TE Buffer: 30 mL
• HiPure Columns: 10
• Column Holders: 3