Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with UD indexes, 96
On demandInvitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with UD indexes, 96
The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries. This Collibri Stranded RNA Library Prep Kit supports fast library preparation within 4.5 hours for mRNA or whole transcriptome libraries with 1–25 ng of mRNA-enriched or ribo depleted RNA sample input. Multiplexing of libraries can be carried out using up to 96 single-indexed or unique dual-indexed primers, enabling single-read or paired-end sequencing. The kit is suitable for various-quality RNA samples, including FFPE samples.
For use with equipment: Illumina NGS instruments.
Features
- Short, automation-friendly workflow—total RNA converted to sequencing-ready library within 4.5 hours
- Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
- Uniform transcript coverage and high transcript detection sensitivity
- High sensitivity of differential gene expression detection
- >98% strand specificity
- Efficient detection of non-coding RNA
Contents
- Collibri Library Prep Kit
- Collibri Library Cleanup Kit
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