Invitrogen™ ERCC RNA Spike-In Mix
On demandInvitrogen™ ERCC RNA Spike-In Mix
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation, in order to measure against defined performance criteria. Up until the design of such universally accepted controls, it has been difficult to execute a thorough investigation of fundamental analytical performance metrics. From the trusted brand of quality RNA reagents, Ambion® ERCC Spike-In Control Mixes are commercially available, pre-formulated blends of 92 transcripts, derived and traceable from NIST-certified DNA plasmids. The transcripts are designed to be 250 to 2,000 nt in length, which mimic natural eukaryotic mRNAs.
Features
- Achieve a standard measure for data comparison across gene expression experiments
- Measure sensitivity (lower limit of detection) and dynamic range of an experiment
- Quantitate differential gene expression
Contents
- 10 µl ERCC RNA Spike-In Mix 1
- 1.75ml Nuclease-free Water
There are no report
You May Also Like
Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL
$ 1,430 $ 1,573
In stock
Applied Biosystems™ ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well
$ 62
In stock
Applied Biosystems™ TaqMan™ Array, Human Protein Kinase, Fast 96-well
$ On demand
On demand
Applied Biosystems™ TaqMan™ SARS-CoV-2 Control Dilution Buffer
$ On demand
On demand
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit, 100 Reactions
$ On demand
On demand
Applied Biosystems™ TaqMan™ Array Human SMAD Signaling Network
$ On demand
On demand
Applied Biosystems™ TaqMan™ Array, Rat Phosphodiesterase, Fast 96-well
$ On demand