Invitrogen™ FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination

Invitrogen™ FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors.
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.


Features

• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success
  
  

Contents

• Alexa Fluor® reactive dyes  (Components A, B, C, and D):5 vials in each of 4 pouches
• Dimethylsulfoxide (DMSO) (Component M): 200 μL
• Dithiothreitol (DTT)  (Component K): 100 μL
• Glycogen, ultrapure, nulcease free (Component L): 20 μL
• Sodium bicarbonate  (Component N): 84 mg
• Water, nuclease free  (Component O): 4.0 mL
• DNA nucleotide mix (Component P): 80 μL
• DNA polymerase I  (Component S): 25 μL
• DNase I (Component R): 15 μL
• Nick translation buffer (Component Q): 250 μL
• SlowFade® Gold antifade reagent (Component J): 2 mL
• Binding buffer (Component E): 6.0 mL
• Wash buffer (Component F): 3.2 mL
• Elution buffer (Component G): 6.0 mL
• Spin columns and collection tubes (Component H):20 columns with tubes
• Collection tubes (Component I): 20 tubes