Invitrogen™ Biotin-14-CTP

Invitrogen™ Biotin-14-CTP

Biotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP (1). The biotinylated RNA can be used as a probe in membrane-based or in situ hybridizations (2). The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template).

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC.

Contents

• Biotin-14-CTP is supplied at 10 mM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.