Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual

Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual

The Ambion® LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit includes sufficient reagents for 20 reactions.
Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion's RNAlater® Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications.

Features

• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: > 1 ml Blood Samples (Large-Scale)
• Starting Material Amount: Up to 10 ml whole blood
• Final Product Type: Cells (Leukocytes)
• Sample Type: Blood

Contents

• 1X PBS pH 7.4: 80 mL
• RNAlater® Solutio: 80 mL
• Rubber Septum Cap: 2
• White Slip Connector: 20
• Transfer Spike: 22
• LeukoLOCK™ Filter: 20