Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with Tempus™ Blood RNA Tubes Green Features

Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with Tempus™ Blood RNA Tubes

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in Tempus™ Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

Features

• Detect total RNA (including miRNA) from Tempus™ Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates
• Fewer resources used and less waste, Less hazardous
• Final Product Type: Lysate
• Purification Target: Lysate, ready for real-time PCR
• Sample Type: Blood

Contents

• Digestion Solution: 20 mL
• Tempus™ Pellet Enhancer: 4 mL
• Tempus™ Wash 1: 150 mL
• Tempus™ Wash 2: 150 mL
• 2X PBS for Tempus™: 50 mL
• Stop Solution: 2 mL
• DNase 1: 200 µL