Invitrogen™ Low Input RiboMinus™ Eukaryote System v2

Invitrogen™ Low Input RiboMinus™ Eukaryote System v2

The Low Input RiboMinus™ Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which introduce bias and select for only part of the transcriptome.
The Low Input RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the Low Input RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

Features

• Magnetic bead-based purification for rapid, seamless purification
• 1-hour workflow
• Low input range of 100 ng–1 µg
• For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)
• Workflow Step: Sample Enrichment
• Species: Human, Mouse, Rat

Contents

• RiboMinus™ Eukaryote Probe Mix v2: 30 μL
• 2X Hybridization Buffer: 1.5 mL
• RiboMinus™ Magnetic Beads: 3 mL
• Binding Solution Concentrate: 6 mL
• Nucleic Acid Binding Beads: 0.15 mL
• Wash Solution Concentrate: 2 mL