Invitrogen™ mirVana™ PARIS™ RNA and Native Protein Purification Kit

Invitrogen™ mirVana™ PARIS™ RNA and Native Protein Purification Kit

The mirVana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample. Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions.

Features

• Isolate small RNA-containing total RNA and native protein from the same sample
• Start with 100–107 cultured cells or up to 100 mg of many mammalian tissues
• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses
• Follow a simple, 30-minute procedure
• Use to correlate mRNA, miRNA or siRNA, and protein levels

Contents

• miRNA Wash Solution I:  30 mL
• Wash Solution 2/3:  50 mL
• Collection Tubes:  80
• Filter Cartridges:  40
• Cell Disruption Buffer:  25 mL
• 2X Denaturing Solution:   25 mL
• Acid-Phenol:Chloroform:  2 × 25 mL
• Elution Solution:  5 mL