Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
20 Reactions
Order number :
K481001
Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit
The GFP Fusion TOPO® TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO® or pcDNA3.1/CT-GFP-TOPO® vectors. Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells. Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.
Contents
- pcDNA3.1/NT-GFP-TOPO®: 20 μL
- 10X PCR Buffer: 100 μL
- dNTP Mix (50 mM dNTPs): 10 μL
- Salt Solution: 50 μL
- Sterile Water: 1 mL
- Control PCR Template: 10 μL
- Control PCR Primers: 10 μL
- Expression Control Plasmid pcDNA3.1/NT-GFP, supercoiled: 10 μL
- Forward Sequencing Primer (GFP Forward Primer): 20μL
- Reverse Sequencing Primer (BGH Reverse Sequencing Primer): 20 μL
- SOC Medium: 6 mL
- TOP10 cells: 21 x 50 μL
- pUC19 Control DNA: 50 μL
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