Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology

Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology

GeneArt® pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt® High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt® pYES1L Vector with Sapphire Technology™.

Features

• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region

Contents

• 5 vials of linear GeneArt™ pYES1L Vector with Sapphire Technology™ at a concentration of 50 ng⁄ml.