Invitrogen™ PichiaPink™ Vector Kit
On demandInvitrogen™ PichiaPink™ Vector Kit
The PichiaPink™ Vector Kit contains the pPINK-HC and pPINK-LC vectors for use with the PichiaPink™ Yeast Expression System. The pPINK vectors are built around the ADE2 gene for complementing the ade2 deletion in the PichiaPink™ strains. You can order the PichiaPink™ Vector Kit to refill your PichiaPink™ Yeast Expression System Kits.
Two Vector Options
The PichiaPink™ Vector Kit comes with two vectors: pPINK-LC (low-copy number) and pPINK-HC (high-copy number). You can choose the vector that works better at producing your protein. Both vectors use the methanol-induced AOX1 promoter to express your protein.
Both pPINK-LC and pPINK-HC vectors are compatible with the PichiaPink™ Secretion Signal Set. Using a 3-way ligation, you can add one of 8 secretion sequences to your gene. The pPINK vectors express proteins intracellularly by default.
Contents
- pPINK-LC vector (low-copy number) (20 µg)
- pPINK-HC vector (high-copy number) (20 µg)
- 5’AOX1 sequencing primer (20 µg)
- 3’CYC3 sequencing primer (20 µg)
- One Shot® TOP10 Electrocomp™ E. coli: C404052
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