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Invitrogen™ EKMax™ Enterokinase, 1000 units

Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1000 Units
Order number : E18002
($) On demand

Invitrogen™ EKMax™ Enterokinase, 1000 units

 

EKMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this peptide sequence.

Application: Removal of fusion tags from recombinant proteins.

Unit Definition: One unit of EKMax™ is the amount of enzyme required to digest 20 µg of a thioredoxin-CAT fusion protein to 90% completion in 16 hours at 37°C. One EKMax™ unit is equivalent to ∼190 trypsinogen activation units.

 

Contents

  • EKMax™: 1.0 mL
  • 10X EKMax™ Reaction Buffer: 3 x 1.5 mL

 

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