Invitrogen™ Poly(A)Purist™ MAG Kit with Manual

Invitrogen™ Poly(A)Purist™ MAG Kit with Manual

Ambion® Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.
The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits. The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes.

Features

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications
• Final Product Type: mRNA
• Purification Target: mRNA
• Sample Type: Total RNA

Contents

• Nuclease-free Water: 5 mL
• Microfuge Tubes: 40 each
• Oligo(dT) MagBeads: 800 µL 
• 2X Binding Solution: 15 mL 
• Wash Solution 1: 40 mL 
• Wash Solution 2: 30 mL 
• THE RNA Storage Solution: 10 mL 
• 5 M Ammonium Acetate: 1 mL 
• Glycogen (5 mg/mL): 100 µL
  
  

Related Accessories 

• A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (AM10026)