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Invitrogen™ pSecTag2/Hygro A, B, & C Mammalian Expression Vectors

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 20 µg
Order number : V91020
$ On demand

Invitrogen™ pSecTag2/Hygro A, B, & C Mammalian Expression Vectors

 

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal.

The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.

The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.

 

Features

  • Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant protein
  • Cytomegalovirus (CMV) promoter for high-level constitutive expression
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
  • C-terminal c-myc epitope for detection with an Anti-myc Antibody
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)

 

Contents

  • 20 µg of each vector and a PSA expression control are supplied supercoiled and lyophilized
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https://www.thermofisher.com/order/catalog/product/V91020?SID=srch-srp-V91020#/V91020?SID=srch-srp-V91020

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