Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit, 25 Preps

Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit, 25 Preps

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold (A35899).

Features

• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

Contents

• Resuspension buffer1 (red): 210 mL
• Lysis buffer[2] (blue): 210 mL   
• Precipitation buffer (yellow): 210 mL  
• Binding buffer: 210 mL   
• Wash buffer 1: 20 mL   
• Wash buffer 2 (concentrate): 10 mL   
• Elution buffer: 6 mL   
• Column assembly 3: 25 columns   
• Syringe filters: 25 filters   
• Syringe plungers: 25 plungers  
• Collection tubes: 25 tubes   
• Quick reference card: 1 card