Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit, 25 Preps
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
25 Preps
Order number :
K210027
Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit, 25 Preps
The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour. Using the HiPure Filter Columns and HiPure Precipitators supplied in the kit, you can purify plasmid DNA without the centrifugation steps required in traditional isolation protocols. The quality of the purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - the most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.
Features
- Simple protocols - bacterial lysate clarification and isopropanol precipitation without centrifugation
- Fast results - from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
- High yields - up to 850 µg from a high-copy plasmid
- Elution Volume: 15 mL
- Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
- Prep Scale: > 200 µg (Large-Scale) Plasmid DNA
Contents
- Resuspension Buffer (R3): 250 mL
- RNase A: 1.5 mL
- Lysis Buffer (L7): 250 mL
- Precipitation Buffer (N3): 250 mL
- Equilibration Buffer (EQ1): 2 × 400 mL
- Wash Buffer (W8): 3 x 500 mL
- Elution Buffer (E4): 400 mL
- TE Buffer (TE): 30 mL
- HiPure Filter Columns: 30 Each
- Column Holders: 5 Each
- PureLink® HiPure Precipitator Module: 25 Each
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