Invitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit, 4 x 96 Preps
On demandInvitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit, 4 x 96 Preps
The PureLink® Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E. coli in as little as 45 minutes.
The PureLink® Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 µg of plasmid DNA from E. coli strains grown in up to 5 ml LB, 3 ml 2xYT, or 3 ml TB. In an automated platform using 1.5 ml E. coli culture grown overnight in a square-well block, typical yields are 5-15 µg plasmid DNA, with an average of 9.4 µg.
The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA. The PureLink® Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning.
Features
- Obtain consistently high yields of high-purity plasmid DNA
- Experience ease of use and better performance due to improved plate design and protocol
- Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms
- For Use With Equipment: Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
- Elution Volume: 50 µlL (centrifuge), 100 µL (vacuum), 150 µL
- Prep Scale: < 100 µg (Small-Scale) Plasmid DNA
Contents
- PureLink™ Pro 96 Resuspension Buffer: 200 mL
- PureLink™ Pro 96 Lysis Buffer: 200 mL
- PureLink™ Pro 96 Neutralization Buffer: 200 mL
- PureLink™ Pro 96 RNase A: 4 mL
- PureLink™ Pro 96 Elution Buffer: 100 mL
- PureLink™ 96 Well Plasmid Filter Plate: 4 each
- PureLink™ Pro 96 Plasmid Clarification Plate: 4 each
- PureLink™ Pro 96 Wash Buffer I: 200 mL
- PureLink™ Pro 96 Wash Buffer II (5X) (in 1 L bottles): 200 mL
- PureLink™ Pro 96 Elution Plate: 4 each
- PureLink™ Pro 96 Growth Block (Square-well): 4 each
- PureLink™ Air Porous Tape: 4 pieces
- Foil Tape: 4 pieces
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